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Impact of "a" determinant mutations on detection of hepatitis B surface antigen (HBsAg) in HBV strains from Chinese patients with occult hepatitis B 期刊论文

编号：

20e013b9-776c-4503-bf3d-16acb3d3d6c5
作者：

Huang, Xiangyan#*^[1]Ma, Chenyun(马晨芸)^[2]Zhang, Qiang^[3];Shi, Qingfen^[4];Huang, Tao^[5];Liu, Chao^[1];Li, Jie^[1];Hollinger, F. Blaine^[6,7,8];
语种：

英文
期刊：

JOURNAL OF MEDICAL VIROLOGY ISSN：0146-6615 2017 年 89 卷 10 期 (1796 - 1803) ; OCT
收录：
关键词：

enzyme assays hepatitis B virus inapparent infection mutation
摘要：

This study was designed to detect mutations that occur within the "a" determinant in the S gene of the hepatitis B virus (HBV) in patients with occult hepatitisB(OHB), and to analyze the influence of these mutations on expression and reactivity of the hepatitis B surface antigen (HBsAg). Twenty-three certified OHB samples were compared to 32 HBsAg positive samples from patients with chronic hepatitis B. The median HBV DNA levels in the OHB group were significantly lower than those in the control group (P < 0.0001). Mutations within the "a" determinant were analyzed by gene amplification and sequencing. This revealed mixed infections in which clones within a sample displayed either different mutations or mutations in association with clones that exhibited wild type amino acid patterns. Sequencing analysis also showed a significant difference between the proportions of amino acid mutations observed in the OHB and control groups. Seven recombinant S (rS) proteins with corresponding OHB mutations and three wild type alleles were expressed and purified in the Pichia pastoris expression system to preserve conformational attributes, and their reactivity analyzed using six commercial HBsAg assays. The OHB sera were HBsAg nonreactive while the rS proteins with corresponding OHB mutations were universally reactive. Thus, we postulate that the reduced binding affinity between mutated HBsAg and its antibody may not be as important in defining OHB as is the effect of specific mutations in the preS/S region of the genome that affect the synthesis and secretion of the S protein and/or the virion.
推荐引用方式
GB/T 7714：

Huang Xiangyan,Ma Chenyun,Zhang Qiang, et al. Impact of "a" determinant mutations on detection of hepatitis B surface antigen (HBsAg) in HBV strains from Chinese patients with occult hepatitis B [J].JOURNAL OF MEDICAL VIROLOGY,2017,89(10):1796-1803.
APA：

Huang Xiangyan,Ma Chenyun,Zhang Qiang,Shi Qingfen,&Hollinger F. Blaine.(2017).Impact of "a" determinant mutations on detection of hepatitis B surface antigen (HBsAg) in HBV strains from Chinese patients with occult hepatitis B .JOURNAL OF MEDICAL VIROLOGY,89(10):1796-1803.
MLA：

Huang Xiangyan, et al. "Impact of "a" determinant mutations on detection of hepatitis B surface antigen (HBsAg) in HBV strains from Chinese patients with occult hepatitis B" .JOURNAL OF MEDICAL VIROLOGY 89,10(2017):1796-1803.
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