Endogenous reactive oxygen species (ROS) control is important for the maintenance of self-renewal of embryonic stem (ES) cells. Although miRNAs have been found to be critically involved in the regulation of the self-renewal, whether miRNAs can regulate the signaling axis to control ROS in ES cells is unclear. Here we show that miR-29b specifically regulates the self-renewal of mouse ES cells in response to ROS generated by antioxidant-free culture. Sirt1 is the direct target of miR-29b and can also make mES cells sensitive to ROS and regulate the self-renewal of mES cells during the response of ROS. We further found that Sirt1 could attenuate the miR-29b function in regulating mES cells' self-renewal in response to ROS. Our results determined that miR-29b-Sirt1 axis regulates self-renewal of mES cells in response to ROS. (C) 2014 Elsevier Inc. All rights reserved.
[1]Tongji Univ, Sch Med, East Hosp, Res Ctr Translat Med, Shanghai 200120, Peoples R China.
[2]Tongji Univ, Sch Med, East Hosp, Dept Prevent Med, Shanghai 200120, Peoples R China.
[3]Tongji Univ, Sch Med, East Hosp, Dept Anesthesiol, Shanghai 200120, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Shanghai Shuguang Hosp, Dept Hosp Infect, Shanghai 201203, Peoples R China.
[5]Fudan Univ, Dept Anesthesiol, Eye Ear Nose & Throat Hosp, Shanghai 200031, Peoples R China.
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