Staphylococcus aureus is an opportunistic human pathogen causing food-borne diseases. Therefore, the goal of the present work was to develop a robust protocol for identification of small amounts of S. aureus DNA in order to establish a rapid, sensitive, and simple method of detect whether this species is present in the stool of patients. A protocol was developed for extraction of DNA by Fe3O4@SiO2 microbeads and subsequent detection of S. aureus-specific DNA sequences by the loop-mediated isothermal amplification (LAMP). The LAMP technique was rapid and sensitive, which allowsed the detect for bacteria at concentrations as low as 10(2) cells/mL. This level of detection was superior to that seen for conventional PCR and real-time PCR. Moreover, the LAMP protocol did not require specialized expensive instrumentation. These features of the novel assay developed here indicate that it may serve as an effective tool for point-of-care diagnosis of acute S. aureus infections.
[1]Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou 510006, Guangdong, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Dept Cardiol, Shanghai 200437, Peoples R China.
[3]Algae Med Sci Co, Guangzhou 51000, Guangdong, Peoples R China.
[4]Jiangsu Univ, Sch Med, Zhenjiang 212013, Jiangsu, Peoples R China.
[5]Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat Collaborat Innova, Zhuzhou 412007, Peoples R China.
[6]Shenzhen Peoples Hosp, Dept Ophthalmol, Shenzhen 518000, Peoples R China.
(8.0+极速模式)