首页 / 院系成果 / 成果详情页

ERK signaling in Bel-7402 hepatocellular carcinoma cells 期刊论文

编号：

1980c5a6-07f3-478e-9c8e-3052ecd47d4d
作者：

Tang, Xun#^[1]Chen, Xianzhen#^[2]Xu, Yanfeng(徐燕丰)#^[3]Qiao, Yongxia^[4];Zhang, Xiao^[1];Wang, Yulan^[1];Guan, Yu(管宇)^[5]Sun, Fenyong*^[1]Wang, Jiayi*^[1,6]
语种：

英文
期刊：

CELLULAR SIGNALLING ISSN：0898-6568 2015 年 27 卷 9 期 (1694 - 1702) ; SEP
收录：
关键词：

Apoptosis Ubiquitination Signaling transduction Tumorigenesis Membrane protein
摘要：

Both Cluster of Differentiation 166 (CD166) and Melanoma Cell Adhesion Molecule (MCAM) play critical roles in maintaining transformative phenotype of Hepatocellular Carcinoma (HCC) cells. However, the relationship between these two membrane proteins remains unknown. Here, we found that CD166 has a positive impact on the expression of MCAM, while MCAM has no feedback on CD166. Tissue microarray analysis (TMA) also showed a positive correlation between CD166 and MCAM. Depletion of CD166-induced anti-carcinogenic phenotype could be reversed by overexpression of MCAM, suggesting MCAM is functional important in the CD166-induced liver tumorigenesis. Furthermore, we found CD166 regulates MCAM mainly through protecting MCAM from ubiquitin-mediated protein degradation. Mechanically, CD166 down-regulated two ubiquitin E3 li-gases, beta TrCP and Smurf1, which play critical roles in the destability of MCAM protein. In addition, overexpression of beta TrCP and Smurf1-reduced transformative phenotype could be partially reversed by MCAM, providing evidence that MCAM is a target of beta TrCP and Smurf1. Moreover, we identified c-Raf/MEK/ERK signaling acts as a downstream effecter of CD166/PI3K/AKT axis to stimulate ubiquitination and destability of beta TrCP and Smurf1. Taken together, we establish a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, beta TrCP and Smurf1, blockage of this signaling cascade may be useful in the treatment of CD166 and MCAM-dependent HCC. (C) 2015 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Tang Xun,Chen Xianzhen,Xu Yanfeng, et al. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and beta TrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells [J].CELLULAR SIGNALLING,2015,27(9):1694-1702.
APA：

Tang Xun,Chen Xianzhen,Xu Yanfeng,Qiao Yongxia,&Wang Jiayi.(2015).CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and beta TrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells .CELLULAR SIGNALLING,27(9):1694-1702.
MLA：

Tang Xun, et al. "CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and beta TrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells" .CELLULAR SIGNALLING 27,9(2015):1694-1702.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：9  下载次数：0

分享到：

浏览次数：9

下载次数：0

打印次数：0