The purpose of this study was to evaluate the effects of exercise training on cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rats. Histopathological analysis, TUNEL assay, and Western blotting were performed on the excised hearts from three groups of Sprague-Dawley rats, which were divided into a sham-operated group, a bilaterally ovariectomized group (OVX), and a bilaterally ovariectomized group that underwent treadmill running exercise for 60 min/day, 5 sessions/wk, for 10 wk (OVX-EX). The abnormal myocardial architecture, cardiac trichome-stained fibrosis and cardiac TUNEL-positive apoptotic cells in ovariectomized rats improved after exercise training. The protein levels of tumor necrosis factor-alpha, tumor necrosis factor receptor 1, Fas ligand, Fas receptors, Fas-associated death domain, activated caspase-8 and activated caspase-3 (Fas receptor-dependent apoptotic pathways), as well as t-Bid, Bad, Bak, Bax, cytosolic cytochrome c, activated caspase-9, and activated caspase-3 (mitochondria- dependent apoptotic pathways) were decreased in the OVX-EX group compared with the OVX group. Exercise training suppressed ovariectomy-induced cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rat models. These findings might indicate a new therapeutic effect for exercise training to prevent cardiac apoptosis in menopausal or bilaterally oophorectomized women.
[1]China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan.
[2]China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan.
[3]Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan.
[4]China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan.
[5]China Med Univ, Grad Inst Rehabil Sci, Dept Phys Therapy, 91 Hsueh Shih Rd, Taichung 40202, Taiwan.
[6]Asia Univ, Dept Psychol, Taichung, Taiwan.
[7]China Med Univ Hosp, Translat Med Res Ctr, Taichung, Taiwan.
[8]China Med Univ, Grad Inst Immunol, Taichung, Taiwan.
[9]China Med Univ Hosp, Dept Neurol, Ctr Neuropsychiat, Taichung, Taiwan.
[10]Chung Shan Med Univ, Ctr Sleep Med, Dept Phys Med & Rehabil, Chung Shan Med Univ Hosp, Taichung, Taiwan.
[11]Univ Taipei, Dept Sports Sci, Taipei, Taiwan.
[12]Asia Univ, Dept Occupat Therapy, Taichung, Taiwan.
[13]Shanghai Univ TCM, Sch Rehabil Sci, Shanghai, Peoples R China.
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